Follow Us

YEAST PLASMİD

YEAST PLASMİD

Zymopure Plasmid kitleri Zymo Research firmamız tarafından geliştirilmiş piyasadaki en üstün özelliklere sahip  MidiPrep, MaxiPrep ve GigaPrep Plasmid İzolasyon kitleridir.


Özellikler:

Ultrapure ve endotoxin free plasmid DNA elde edilir.

Precipitation ve çok basamaklı sentrifüj adımları gerektirmez.

İşlem süresi 20 dakikadan kısadır.

Kitlerle sentrifüj veya vakuma dayalı    protokol uygulanabilir.

En düşük elution volümünde, en yüksek konsantrasyonda plasmid DNA elde edilir.

En düşük endotoxin seviyesine sahip plasmid DNA transfeksiyon restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing ve diğer sensitive downstream aplikasyonları için uygundur.

 

  •   Zymopure Kitleri

 

D4200

ZymoPURE Midiprep Kit (25 Preps)

D4201

ZymoPURE Midiprep Kit (50 Preps)

D4202

ZymoPURE Maxiprep Kit (10 Preps)

D4203

ZymoPURE Maxiprep Kit (20 Preps)

D4204

ZymoPURE Gigaprep Kit (5 Preps)


 

 

 


Yield, concentration and transfection efficiency for plasmid DNA isolated using the ZymoPURE Maxiprep kit compared to two separate kits from Supplier Q. Plasmid DNA (pGL3®) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate). HeLa cells cultured in a 96-well plate were transfected with 100 ng of pGL3® Luciferase Reporter Vector using Lipofectamine® 2000 and luciferase expression was measured 48 hours later with the ONE-Glo Luciferase Assay System and Veritas Microplate Luminometer. Shown are means ± SEM of 8 transfections.

Plasmid DNA yield and concentration from the ZymoPure Maxiprep kit compared to other major suppliers. Plasmid DNA (pGEM®) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate). One (1) µl of eluted plasmid DNA was visualized post agarose gel electrophoresis. M, ZR 1 kb DNA Marker (Zymo Research).

Plasmid Miniprep Kitleri
pellet-free modified alkaline lysis method

Zyppy™ Plasmid Kitleri pellet-free modified alkaline lysis metodunu kullanarak bacterial culture centrifugation ve resuspension adımlarını elemine eder.

APPLICATION

  • Plasmid Recovery from E. Coli

FEATURES

  • Direct Culture Input
  • Colored Buffers
  • Endotoxin Free

 

Format: Spin Column

Binding Capacity: 25 µg/prep.

Elution Volume: ≥ 30 µl

Culture Input: 600 µl - 3 ml

Typical Yield (high copy plasmid):

2 - 15 µg

DNA Size Limit: ≤ 25 kb

Processing Time: 8 min.

 

D4036

Zyppy™ Plasmid Miniprep Kit  (50 Preps) 

D4019

Zyppy™ Plasmid Miniprep Kit (100 Preps) 

D4020

Zyppy™ Plasmid Miniprep Kit (400 Preps)

D4037

Zyppy™ Plasmid Miniprep Kit (800 Preps)

D4041

Zyppy-96™ Plasmid Kit (2 x 96 Preps) 

D4042

Zyppy-96™ Plasmid Kit (4 x 96 Preps) 

D4043

Zyppy-96™ Plasmid Kit (8 x 96 Preps) 

D4100

Zyppy-96™ Plasmid MagPrep Kit (2 x 96 Preps)

D4101

Zyppy-96™ Plasmid MagPrep Kit (4 x 96 Preps)

D4102

Zyppy-96™ Plasmid MagPrep Kit (8 x 96 Preps)

D4025

Zyppy™ Plasmid Midiprep Kit (25 Preps)

D4026

Zyppy™ Plasmid Midiprep Kit (50 Preps) w/ Zymo Spin V-E

D4027

Zyppy™ Plasmid Maxiprep Kit (10 Preps) w/ Zymo Spin VI

D4028

Zyppy™ Plasmid Maxiprep Kit (20 Preps) w/ Zymo Spin VI

D4015

ZR Plasmid Miniprep™-Classic Kit (100 Preps)

D4016

ZR Plasmid Miniprep™-Classic Kit (400 Preps)

D4054

ZR Plasmid Miniprep™-Classic Kit (800 Preps)

Ürün Kodu
Ürün Adı
Ambalajı
Markası
YEAST PLASMID
D2001Zymoprep™ Yeast Plasmid Miniprep I100 PrepZymo Research
D2004Zymoprep™ Yeast Plasmid Miniprep II50 PrepZymo Research