YEAST PLASMİD
Zymopure Plasmid kitleri Zymo Research firmamız tarafından geliştirilmiş piyasadaki en üstün özelliklere sahip MidiPrep, MaxiPrep ve GigaPrep Plasmid İzolasyon kitleridir. Özellikler: Ultrapure ve endotoxin free plasmid DNA elde edilir. Precipitation ve çok basamaklı sentrifüj adımları gerektirmez. İşlem süresi 20 dakikadan kısadır. Kitlerle sentrifüj veya vakuma dayalı protokol uygulanabilir. En düşük elution volümünde, en yüksek konsantrasyonda plasmid DNA elde edilir. En düşük endotoxin seviyesine sahip plasmid DNA transfeksiyon restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing ve diğer sensitive downstream aplikasyonları için uygundur. |
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Yield, concentration and transfection efficiency for plasmid DNA isolated using the ZymoPURE™ Maxiprep kit compared to two separate kits from Supplier Q. Plasmid DNA (pGL3®) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate). HeLa cells cultured in a 96-well plate were transfected with 100 ng of pGL3® Luciferase Reporter Vector using Lipofectamine® 2000 and luciferase expression was measured 48 hours later with the ONE-Glo™ Luciferase Assay System and Veritas™ Microplate Luminometer. Shown are means ± SEM of 8 transfections.
Plasmid DNA yield and concentration from the ZymoPure™ Maxiprep kit compared to other major suppliers. Plasmid DNA (pGEM®) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate). One (1) µl of eluted plasmid DNA was visualized post agarose gel electrophoresis. M, ZR 1 kb DNA Marker (Zymo Research).
Plasmid Miniprep Kitleri
pellet-free modified alkaline lysis method
Zyppy™ Plasmid Kitleri pellet-free modified alkaline lysis metodunu kullanarak bacterial culture centrifugation ve resuspension adımlarını elemine eder. APPLICATION
FEATURES
Format: Spin Column Binding Capacity: 25 µg/prep. Elution Volume: ≥ 30 µl Culture Input: 600 µl - 3 ml Typical Yield (high copy plasmid): 2 - 15 µg DNA Size Limit: ≤ 25 kb Processing Time: 8 min. |
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