Follow Us

Zyppy Plasmid Miniprep Kit

Zyppy Plasmid Miniprep Kit

🔊 Zyppy®️ Plazmit Miniprep Kit;

👩🏻‍🔬 Kültürden Doğrudan Plazmit DNA İzolasyonu

Zyppy®️ Plazmit Miniprep Kiti, klasik plazmit hazırlama prosedürlerinde yaygın olan bakteri kültürü santrifüjünü ve yeniden süspanse etme adımlarını atlayan, pelet içermeyen, modifiye edilmiş bir alkalin liziz yöntemine sahiptir. Benzersiz formüle edilmiş 7X Lysis Buffer'ı doğrudan bakteri kültürünüze ekleyin, nötralize edin ve ardından kitin içerisindeki Zymo-Spin™️ Kolon teknolojisini kullanarak saflaştırın. Ek olarak, kitte bulunan renkli bufferlar, eksiksiz bakteri hücre lizizi ve nötralizasyonunun hatasız görselleştirilmesine ve gerçekleştirmesini sağlar. Elde edilen plazmit DNA, yüksek kalitededir ve hassas downstream uygulamalar için hazırdır. Klonlama, dizileme ve transfeksiyon için 8 dakikada yüksek kaliteli plazmit DNA hazır!

✅• 1. Doğrudan liziz bufferını doğrudan bakteri kültürüne ekleyin.
✅• 2. Nötralize edin.
✅• 3. Bağlayın, yıkayın, saflaştırın.

🧬 Teklif ve bilgi almak için: [email protected] mail adresimize, mail atabilirsiniz. 

 


Yield, concentration and transfection efficiency for plasmid DNA isolated using the ZymoPURE Maxiprep kit compared to two separate kits from Supplier Q. Plasmid DNA (pGL3®) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate). HeLa cells cultured in a 96-well plate were transfected with 100 ng of pGL3® Luciferase Reporter Vector using Lipofectamine® 2000 and luciferase expression was measured 48 hours later with the ONE-Glo Luciferase Assay System and Veritas Microplate Luminometer. Shown are means ± SEM of 8 transfections.

Plasmid DNA yield and concentration from the ZymoPure Maxiprep kit compared to other major suppliers. Plasmid DNA (pGEM®) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate). One (1) µl of eluted plasmid DNA was visualized post agarose gel electrophoresis. M, ZR 1 kb DNA Marker (Zymo Research).

Plasmid Miniprep Kitleri
pellet-free modified alkaline lysis method

Zyppy™ Plasmid Kitleri pellet-free modified alkaline lysis metodunu kullanarak bacterial culture centrifugation ve resuspension adımlarını elemine eder.

APPLICATION

  • Plasmid Recovery from E. Coli

FEATURES

  • Direct Culture Input
  • Colored Buffers
  • Endotoxin Free

 

Format: Spin Column

Binding Capacity: 25 µg/prep.

Elution Volume: ≥ 30 µl

Culture Input: 600 µl - 3 ml

Typical Yield (high copy plasmid):

2 - 15 µg

DNA Size Limit: ≤ 25 kb

Processing Time: 8 min.

 

D4036

Zyppy™ Plasmid Miniprep Kit  (50 Preps) 

D4019

Zyppy™ Plasmid Miniprep Kit (100 Preps) 

D4020

Zyppy™ Plasmid Miniprep Kit (400 Preps)

D4037

Zyppy™ Plasmid Miniprep Kit (800 Preps)

D4041

Zyppy-96™ Plasmid Kit (2 x 96 Preps) 

D4042

Zyppy-96™ Plasmid Kit (4 x 96 Preps) 

D4043

Zyppy-96™ Plasmid Kit (8 x 96 Preps) 

D4100

Zyppy-96™ Plasmid MagPrep Kit (2 x 96 Preps)

D4101

Zyppy-96™ Plasmid MagPrep Kit (4 x 96 Preps)

D4102

Zyppy-96™ Plasmid MagPrep Kit (8 x 96 Preps)

D4025

Zyppy™ Plasmid Midiprep Kit (25 Preps)

D4026

Zyppy™ Plasmid Midiprep Kit (50 Preps) w/ Zymo Spin V-E

D4027

Zyppy™ Plasmid Maxiprep Kit (10 Preps) w/ Zymo Spin VI

D4028

Zyppy™ Plasmid Maxiprep Kit (20 Preps) w/ Zymo Spin VI

D4015

ZR Plasmid Miniprep™-Classic Kit (100 Preps)

D4016

ZR Plasmid Miniprep™-Classic Kit (400 Preps)

D4054

ZR Plasmid Miniprep™-Classic Kit (800 Preps)

Ürün Kodu
Ürün Adı
Ambalajı
Markası
Zyppy Plasmid Miniprep Kit
D4019Zyppy™ Plasmid Miniprep Kit100 PrepsZymo Research https://youtu.be/KEqQKDiVtK4
D4020Zyppy™ Plasmid Miniprep Kit400 PrepsZymo Research https://youtu.be/KEqQKDiVtK4
D4036Zyppy™ Plasmid Miniprep Kit50 PrepsZymo Research https://youtu.be/KEqQKDiVtK4
D4037Zyppy™ Plasmid Miniprep Kit800 PrepsZymo Research https://youtu.be/KEqQKDiVtK4
 Zyppy-96 Plasmid Kit
D4041Zyppy-96™ Plasmid Kit2 x 96 PrepsZymo Research
D4042Zyppy-96™ Plasmid Kit4 x 96 PrepsZymo Research https://youtu.be/KEqQKDiVtK4
D4043Zyppy-96™ Plasmid Kit8 x 96 PrepsZymo Researchhttps://youtu.be/KEqQKDiVtK4
Zyppy-96 Plasmid MagBead Miniprep Kit
D4100Zyppy-96™ Plasmid MagPrep Miniprep Kit2 x 96 PrepsZymo Research
D4101Zyppy-96™ Plasmid MagBead Miniprep Kit4 x 96 Preps
Zymo Research https://youtu.be/KEqQKDiVtK4
D4102Zyppy-96™ Plasmid MagBead Miniprep Kit8 x 96 Preps
Zymo Research https://youtu.be/KEqQKDiVtK4