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Next-Gen Sequencing Library Prep

Next-Gen Sequencing Library Prep

TEKNİK ÖZELLİKLER

DNA daki global 5-methylcytosine oranını ölçen kolormetrik elisa kitleri

 

Sonuçlar % metilasyon olarak elde edilir.

 

DNA bisülfit modifikasyonu gerektirmez

 

Numune olarak genomic DNA, plasmid DNA, PCR amplification products veya DNA fregmantları kullanılır.

 

10-200 ng DNA yeterlidir.

 

Highly sensitive,  % 0.5 ‘e kadar metilasyon tespit eder.

 

Kit 5-mC spesifik ve sensitive Anti-5-Methylcytosine monoclonal antikor içerir.

 

Kit standart eğrisini oluşturmak için gerekli standart ve kontrolleri içerir.

İşlem süresi 3 saattir.

 

                      Sonuçlar 450 nm ‘de kolormetrik olarak ölçülür.

 

 

 

Global DNA Methylation – LINE-1 Assay compares 5-mC levels across cell lines and treatment conditions


Ürün Kodu
Ürün Adı
Ambalajı
Markası

D5455Pico Methyl-Seq™ Library Prep Kit10 PrepZymo Researchdatasheet_pico-methyl-seq----library-prep-kit-poster_56811dd0610f9.pdf
D5456Pico Methyl-Seq™ Library Prep Kit25 PrepZymo Researchdatasheet_pico-methyl-seq----library-prep-kit-protocol_56811db276520.pdf
D5450RRHP™ 5-hmC Library Prep Kit12 PrepZymo Researchdatasheet_rrhp----5-hmc-library-prep-kit-poster-1_56811df320462.pdf
D5451RRHP™ 5-hmC Library Prep Kit25 PrepZymo Researchdatasheet_rrhp----5-hmc-library-prep-kit-poster-2_56811e2d51787.pdfdatasheet_rrhp----5-hmc-library-prep-kit-protocol_56811e0ec69c7.pdf
55019Hydroxymethyl Collector™-Seq Kit25 RxnsActive Motifdatasheet_hydroxymethyl-collector------seq-kit-protocol_56811e636cb54.pdf
58001GenoMatrix™ Whole Genome Amplification Kit50 PrepActive MotifDATASH~1.PDF

eNGen DNA Library Preparation Kit
Bioline
53041ChIP-IT® ChIP-Seq10 librariesActive Motifdatasheet_chip-it---chip-seq-protocol_56811e90170e0.pdf
52043ChIP-exo Kit12 rxnsActive Motif
53048ChIP-Bis-Seq Kit10 librariesActive Motifdatasheet_chip-exo-kit-protocol_56811ea56d210.pdf
54000NOMe-Seq10 rxnsActive Motifdatasheet_nome-seq-protocol_56811ecd8913e.pdfdatasheet_nome-seq-whole-genome-analysis-guidelines_56811eda19a00.pdf
D4080Select-a-Size DNA Clean & Concentrator™25 PrepZymo ResearchDATASH~1.PDF