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Recombinant Protein

Recombinant Protein

What Is A Recombinant Protein

A Recombinant protein is a protein produced artificially by an expression host organism, aka expression system, that is transfected with a recombinant gene (target gene) isolated from another organism. The purpose is to produce the target gene encoded protein in large quantities for medical, research and academic uses.

Expression Systems

There are several common expression systems for producing recombinant proteins, such as E. Coli, Yeast, Insect Cell lines such as Sf21, mammalian cells such as CHO, HEK, and human cell lines. These expression systems act as the factory for recombinant protein production, where the cellular mechanisms involved in protein production uses the recombinant gene in the vector as blueprint to mass produce the target protein. E. Coli is the first organism to be used as an expression system. It is easy to use however cannot produce protein with post-translational modification (PTM) because it lacks the cellular mechanisms of eukaryotic cells which is necessary for PTM of proteins. Thus for producing simple proteins and for applications that do not require proteins to have PTM, E. Coli is a sufficient and convenient expression system however for more complex applications, more advanced expression systems are required.

Expression Vectors

A vector is a tool for manipulating DNA. It is the transport vehicle for the gene of the protein of interest. There are many types of vectors, some common vectors are plasmids and reverse transcription viruses. The DNA that encodes the protein is first synthesized in vitro, or cloned from native host DNA templates, then inserted into the vector DNA. The host organism is processed to take up vectors. These vectors, once inside the corresponding host cells, will be transcribed and translated into proteins.

Ürün KoduÜrün AdıAmbalajıMarkasıDöküman
R00121-1


Recombinant Human FGF2 Protein


100μg/vial
BOSTER
R00121-1_DS.pdf
R00804


Recombinant Human OSM Protein 


100μg/vial
BOSTER

R00804_DS.pdf
R00609


Recombinant Human CNTF Protein


100μg/vial
BOSTER

R00609_DS.pdf
R01931


Recombinant Human FGF7 Protein


100μg/vial
BOSTER
R01931_DS.pdf
R01400-1


Recombinant Mouse LIF Protein


100μg/vial
BOSTER

R01400-1_DS.pdf
R01709

Recombinant Human FGF10 Protein


100μg/vial

BOSTER

R01709_DS.pdf
R00393

Recombinant Human IFN Gamma Protein


100μg/vial

BOSTER
R00393_DS.pdf
R00002

Recombinant Human TNF Alpha Protein



100μg/vial

BOSTER

R00002_DS.pdf